Figure 1

Synergic effect of FA and NLCs on ERK1/2, c-Myc expression levels. Representative immunoblots of (a) ERK and p-ERK and (b) c-Myc expression levels in U-87 MG human cell lines in the absence or in the presence of 36 µM of FA, blank NLCs or FA-NLCs, for 24 h. β-tubulin has been used to normalize protein expression levels. Cropped images of p-ERK, ERK and β-Tubulin have been obtained from the same filter after sequential exposure to the correspondent primary antibodies to analyse the changes in expression levels of phosphorylated protein versus the un-phosphorylated ones. Cropped images belonging to the same filter, obtained from other Western Blot, are also shown for c-Myc and β-Tubulin. Densitometric analysis of (c) phosphorylated ERK1/2 expression levels and (d) c-Myc expression levels in response to the treatments, when compared with untreated ones used as controls. The results (c,d) are reported as the mean ± S.D. of the values of four separated experiments performed in triplicate. Significant differences: **p < 0.01 DMSO vs NLC and FA-NLC; ***p < 0.001 samples vs controls; ^§§§, ^$$$p < 0.001 FA-NLC vs DMSO and vs FA, respectively.