Figure 4
Relationship between gene expression and promoter chromatin accessibility in chicken lung for different ATAC-seq protocol iterations. (a) All genes from the Galgal5 Ensembl 94 annotation were sorted by expression (normalized VST counts) and their promoters (±2 kb from TSS) were classified as either open (green) or closed (black), based on overlap with ATAC-seq peaks from each chicken lung library (minimum overlap of 1 bp). RNA-seq data were procured from different individuals than the ATAC-seq data. (b) Normalized read depth of ATAC-seq and DNase-seq libraries at the TSS of the highly expressed gene cystatin C (CST3).
