Figure 6
ATAC-seq libraries generated for three adult pig tissues. (a) Summary of library details, read depth used for calling peaks, and overlap (minimum 1 bp) with active histone modifications. (b) Fraction of reads in peaks (FRiP) that overlapped (minimum 1 bp) promoters (2 kb upstream of TSS) and distal elements (>2 kb from TSS). (c) ATAC-seq quality metrics: estimated library size (“Lib size”; purple), percentage of reads that mapped to mitochondrial DNA (“% Mito”; blue), enrichment of signal at promoters (±2 kb from TSS; orange; Ensembl Sscrofa10.2.89 annotation; minimum 1 bp overlap of ATAC-seq peak with promoter), and FRiP (green). Desirable values are shaded darker, with the scale starting at 0 (white) and ending at the maximum value. For the percent mitochondrial reads, anything below 5% is shaded blue. For FRiP, all values above 10% are shaded green. All values were determined from 5 million random aligned reads.
