Table 4 Strains and plasmids used in this study.
From: New Therapeutic Candidates for the Treatment of Malassezia pachydermatis -Associated Infections
Strain | Description | Reference |
|---|---|---|
Malassezia pachydermatis CBS 1879 | Reference strain. Genome Sequenced: NCBI: txid 77020. | |
Malassezia furfur CBS 1878 | Reference strain | |
Atypical Malassezia furfur 4DS | Reference strain | |
Malassezia sympodialis CBS 7222 | Reference strain | |
Candida krusei ATCC 6258 | Reference strain | |
Candida parapsilosis ATCC 22019 | Reference strain | |
Malassezia pachydermatis C1 | Canine isolate from the collection of the Cellular and Molecular research group of Microorganisms Pathogens (CeMoP, acronym in Spanish). From ears of a 2-years old female cocker spaniel dog. | |
Malassezia pachydermatis N1 | Canine isolation from the collection of the CeMoP. From ears of a 9-years old female cocker spaniel dog. | |
Malassezia pachydermatis M1 | Canine isolation from the collection of the CeMoP. From the ears of a male 1-year old Shih-Tzu dog. | |
DH5α-pUC57-mpsdh | Escherichia coli DH5α strain transformed with pUC57-mpsdh | This study |
DH5α-pET6xHN-C-mpsdh | Escherichia coli DH5α strain transformed with pET6xHN-C-mpsdh | This study |
BL21-pET6xHN-C-mpsdh | Strain Escherichia coli BL21(DE3) transformed with pET6xHN-C-mpsdh | This study |
BL21-pET6xHN-N-mphcs | Strain Escherichia coli BL21(DE3) transformed with pET6xHN-N-mphcs | This study |
BL21-pET6xHN-N-mphsd | Strain Escherichia coli BL21(DE3) transformed with pET6xHN-N-mphsd | This study |
pET6xHN-C | Plasmid containing an IPTG inducible promoter system (T7/lac promoter) for high-level expression, a C-terminal 6xHN tag and conferring ampicillin resistance | |
pET6xHN-N | Plasmid containing an IPTG inducible promoter system (T7/lac promoter) for high-level expression, an N-terminal 6xHN tag and conferring ampicillin resistance | |
pUC57-mpsdh | Plasmid pUC57 containing a synthetic expression cassette encoding the enzyme saccharopine dehydrogenase (MpSDH) of M. pachydermatis flanked by NcoI and NotI restriction sites and conferring ampicillin resistance. | This study |
pET6xHN-C-mpsdh | Plasmid pET6xHN-C containing a synthetic expression cassette encoding the enzyme saccharopine dehydrogenase (MpSDH) of M. pachydermatis flanked by NcoI and NotI restriction sites, conferring ampicillin resistance and with C-terminal 6xHN tag. | This study |
pET6xHN-N-mphcs | Plasmid pET6xHN-N containing an amplified expression cassette encoding the enzyme homocitrate synthase (MpHCS) of M. pachydermatis flanked by HCSF and HCSR primers sites, conferring ampicillin resistance and with C-terminal 6xHN tag. | This study |
pET6xHN-N-mphsd | Plasmid pET6xHN-N containing an amplified expression cassette encoding the enzyme homoserine dehydrogenase (MpHSD) of M. pachydermatis flanked by HSDF and HSDR primer sites, conferring ampicillin resistance and with C-terminal 6xHN tag. | This study |
