Table 3 Recommended reference genes according to the experimental condition.

From: Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae)

Experimental condition

Reference genes

Developmental stages

EF1-α

18S

RPL12

Sex

RPS23

RPL12

 

Low temperature

GAPDH

α-TUB

 

High temperature

α-TUB

RPS23

 

All temperatures

α-TUB

GAPDH

 

Starvation

RPL12

α-TUB

 

dsRNA

α-TUB

RPL12