Table 4 Primers used for dsRNA synthesis.

From: Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae)

Primer

Sequence 5′−3′

Size (bp)

RpII140-Ae-ds-F

TAATACGACTCACTATAGGGAGCGGGATGAATCTCACAGT

334

RpII140-Ae-ds-R

TAATACGACTCACTATAGGGGCGTCAGATGGACATTATCG

Prosα-2-Ae-ds-F

TAATACGACTCACTATAGGGCGCAACGGAAAATAAACACA

338

Prosα-2-Ae-ds-R

TAATACGACTCACTATAGGGTCCATGCAAAGTAAGCTCCA

  1. Bold letters represent the T7 promoter sequence.
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