Figure 2 | Scientific Reports

Figure 2

From: The unprecedented membrane deformation of the human nuclear envelope, in a magnetic field, indicates formation of nuclear membrane invaginations

Figure 2

Thermotropism of hNM lipid extract membranes and POPC controls. (A) Selected 2H-NMR spectra of reconstituted human nuclear lipid membrane extracts (NLE) mixed with 2H31-POPC and pure 2H31-POPC (POPC) vesicles during a thermal variation (25 °C down to −20 °C and back) and recorded after temperature stabilization. Spectra were obtained after Fourier transformation of solid-echo type experiments accumulated for 2–18 k transients. The entire thermal variation is shown in Supplementary Fig. 5. Details for experimental parameters and data treatment are in the methods section. (B) Thermal variation of the first spectral moment (reporting membrane fluidity), M1, from 2H-NMR spectra of A and Supplementary Fig. 3 of reconstituted NLE (□), and control POPC vesicles (■). The double y-axis plots twice the fatty acid chain order parameter to depict rigid chains (solid-ordered membranes) when 2 SCD chain = 1 and fully disordered systems (liquid-like) when 2 SCD chain = 0.

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