Figure 1
aSyn Fibrils are similarly endocyted in primary neuronal cultures from different brain regions. Primary neuronal cultures from WT or SNCA−/− embryos were exposed to 100 nM Fibrils at DIV7 and observed at DIV14. (a) Confocal imaging in fixed cells revealed that aSyn Fibrils were present in the neuronal lumen, as evidenced by localization of exogenously added aSyn Fibrils (green) in neuronal somas stained with TUJ1 (red) in Hip neurons and (b) in Cx neurons. Scale bar represents 50 µm. (c) aSyn Fibrils are endocyted by Str, Cx, Hip and HipSNCA−/− neurons, as evidenced by epifluorescence imaging. aSyn Fibrils fluorescence in red is superimposed to phase contrast images (grey). Scale bar represents 20 µm. (d) Quantification of the mean aSyn Fibrils signal in neuronal somas in live cell cultures. 5 to 14 replicates from 2 to 3 individual experiments. Means+/− 95% confidence interval (CI95) are shown. (e) aSyn Fibrils are taken up by astrocytes and microglia, as evidenced by epifluorescence imaging. Scale bar represents 100 µm. (f) Quantification of the percentage of neuronal (MAP2 positive) or glial cells (GFAP and CD68 positive) containing aSyn Fibrils in fixed primary cultures. 5 to 8 replicates from 3 individual experiments. Means +/− CI95 are shown.
