Figure 2

Iba-1+ cell number in the photoreceptor outer segment layer (OS), outer plexiform layer (OPL) and inner plexiform layer (IPL) and cell body area of Iba-1+ cells in the OPL, IPL and the nerve fiber-ganglion cell layer (NFL-GCL) at different times after laser-induced ocular hypertension (OHT). (a–c) Variation over time in the number of Iba-1+ cells. Histograms compare the mean (±SD) number of Iba-1+ cells per area of 0.1502 mm² at 1, 3, 5, 8 and 15 d between eyes with ocular hypertension (OHT) and contralateral eyes in OS (a), OPL (b) and IPL (c). (d,e) Immunohistochemical images of anti-Iba-1-stained retinal whole-mounts (d: naïve; e: OHT and contralateral). OS (a,e) In OHT eyes, the number of Iba-1+ cells increased from 1 to 5 d, then decreased at 8 and 15 d. In contralateral eyes, the number of Iba-1+ cells increased from 1 to 3 d, then decreased slightly until 15 d. OPL and IPL (b,c,e) In OHT eyes, the largest increase in the number of microglial cells from 1 d occurred at 3 and 5 d. The number decreased from 5 d until 8 d and 15 d. (f–h) Variation over time in the cell body area of Iba-1+ cells after laser-induced OHT. Histograms show mean (±SD) cell body area of Iba-1+ cells in OHT eyes and contralateral eyes at 1, 3, 5, 8 and 15 d in OPL (f), IPL (g) and NFL-GCL (h). OPL-IPL (e,f,g) The cell body area in OHT eyes was significantly larger at 1 d, then significantly decrease at 5, 8 or 15 d. In contralateral eyes, the cell body area was greatest at 3 d, and then it decreased significantly at 8 and 15 d. NFL/GCL (h) In OHT eyes, the cell body area was largest at 1 d, and then it progressively decreased at 5, 8 and 15 d. In contralateral eyes, the cell body area was greater at 3 d, and then it decreased such that it was significantly smaller at 15 d than at 3 d. The dotted line indicates the mean values in naïve eyes. *p < 0.05, **p < 0.01.