Figure 5
From: Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma
P2RY12 expression in ocular hypertension (OHT) eyes in the photoreceptor outer segment layer (OS) at different times after laser-induced OHT (1, 3, 5, 8 and 15 d). Double immunostaining: Iba-1 (A,D,G,J,M), P2RY12 (B,E,H,K,N), merge (C,F,I,L,O). Retinal whole-mounts. At 1 and 15 d after laser induction, most Iba-1+ cells showed strong P2RY12 expression in OS. At 3 and 5 d, P2RY12 expression in Iba-1+ cells was substantially down-regulated in this layer; it increased slightly at 8 d (arrowheads), reaching values similar to those of naïve eyes at 15 d. Rounded cells were P2RY12- (arrow) (inset A–C).
