Figure 8
From: Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma
P2RY12 expression in ocular hypertension (OHT) eyes in the nerve fiber-ganglion cell layer (NFL-GCL) at different times after laser-induced OHT (1, 3, 5, 8 and 15 d). Double immunostaining: Iba-1 (A,D,G,J,M), P2RY12 (B,E,H,K,N), merge (C,F,I,L,O). Retinal whole-mounts. At 1 d, most Iba-1+ cells stained intensely as P2RY12+, while only a few rounded cells (inset A-C arrows) and perivascular cells (arrow) were P2RY12-. At 3 and 5 d, P2RY12 expression in Iba-1 + cells was dramatically reduced in this layer, then it increased slightly at 8 d (arrowheads) to reach values similar to those in naïve eyes at 15 d. At 15 d, a few ramified Iba-1+ cells did not express P2RY12 (arrows).
