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Figure 1

From: Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum

Figure 1

Characterization of the nuclear CCAAT-binding complex of F. graminearum. (a) Nuclear localization of Fct1-GFP and Fct2-GFP. FCT1c-r/FCT2c-r strains carrying both Fct1-GFP or Fct2-GFP and hH1-RFP were used for the colocalization study. Scale bar = 20 µm. (b) Consensus binding sequence identified via the PBM assay. Consensus sequences that robustly bound to the Fct2-DsRed fusion protein. (c) The effects of positional mutations in CCAATC. To visualize the effects of the mutations on the binding intensities in the consensus binding motif, the average binding intensities (+) of the probes containing the core consensus 6-mer binding motif CCAATC relative to those of probes with mutations at each position (bar) are plotted. (d) Yeast two-hybrid analysis of the interaction between Fct1 and Fct2. The plasmid pairs pDHB1-Fct1/pAI-Alg5 and pDHB1-Fct1/pDL2-Alg5 served as positive and negative controls, respectively. The growth of the transformed yeast was assayed on synthetic dextrose medium lacking Leu and Trp (SD-LT) or Leu, Trp, His, and Ade (SD-LTHA). The columns in each panel represent serial decimal dilutions.

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