Figure 4
Effect of Rhein on HDAC activity and G2/M cell cycle phase regulation, p53 and p21 abundance and proliferation markers. (A) Representative blots and quantification showing Rhein-mediated increase of p53 and p21 abundance (n = 4). (B) Representative blots and quantification showing Rhein-driven p53 stabilization by increased HDAC inhibition-mediated acetylation at Lys382 (n = 4). 1 mM sodium butyrate (SB) was used as reference HDAC inhibitor. (C) Representative blots and quantification showing Rhein-mediated increase of p53 abundance (n = 4) under conditions described in (B). (D) Representative blots and quantification showing Rhein-mediated increase of p21 abundance (n = 4) under conditions described in (B). Antibodies in (C,D) were probed to the same membrane. (E) Enzymatic HDAC activity is inhibited in the presence of Rhein. Graph showing HDAC activity after 30 min treatment of normal cell lysates with Rhein or SB (n = 4). All data are presented as mean ± SD. One-way-ANOVA with post-hoc Sidak’s multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001, as indicated.
