Figure 6
From: Tenascin-C inactivation impacts lung structure and function beyond lung development
Lung histology in adult TNC KO and WT mice. Representative images from lung sections of TNC KO and WT P90 animals. Five µm lung sections were stained with the following staining: Panel a,b: hematoxylin-eosin staining for morphological studies; Panel c,d: resorcin-fuchsin staining for elastin (dark purple); Panel e-h: Masson Trichrome staining for collagen (green). N = 6 animals/genotype. Magnification: 20x for a-f, and 40x for g,h. Panel i: quantification of collagen area on Masson trichrome staining (40×) using ImageJ; results are expressed as collagen area per length of basement membrane; N = 6 animals/genotype. Panel j,k: representative results of elastin protein detection in whole lung lysates from adult TNC KO and WT animals by Western blots; quantification is shown in histogram; N = 5 animals/genotype. Cropped blots are displayed. Full length blots are presented in Supplementary Figure S2.
