Figure 2 | Scientific Reports

Figure 2

From: Proteogenomic analysis of granulocyte macrophage colony- stimulating factor autoantibodies in the blood of a patient with autoimmune pulmonary alveolar proteinosis

Figure 2

Proteomic platform for the identification of peptides derived from purified GM-CSF autoantibodies (GMAbs). Serum from a patient was processed for the purification of IgG, followed by isolation of GM-CSF autoantibodies using a GM-CSF-coupled column. Isolated GMAbs were loaded on SDS-PAGE. The band for the IgG-heavy chain was cut and subjected to in-gel digestion using trypsin. The resultant peptides were purified with a C18 column and subjected to nano-elute UH-PLC-coupled TIMS-TOF instrument MS. Raw MS/MS data were subjected to analysis using PEAKS Studio 8.5 for data processing.

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