Figure 5 | Scientific Reports

Figure 5

From: Isorhamnetin 3-O-neohesperidoside promotes the resorption of crown-covered bone during tooth eruption by osteoclastogenesis

Figure 5

Isorhamnetin 3-O-neohesperidoside promoted bovine bone slice resorption in vitro. Immunofluorescence showed the formation of podosomal actin rings (white arrows) in both untreated OCs and 50 μM isorhamnetin 3-O-neohesperidoside-treated OCs (B). (C)More podosome actin rings were formed in the 50 μM isorhamnetin 3-O-neohesperidoside-treated group. BMMs were treated with isorhamnetin 3-O-neohesperidoside (0 and 50 μM) and RANKL (50 ng/ml) for 9 days. (D) Scanning electron microscopy (SEM) showed only a few resorption pits (black arrows) were observed on the bovine bone slice in the untreated group. (F) More resorption pits were observed in the 50 μM isorhamnetin 3-O-neohesperidoside-treated group (E) than in the untreated group (*p < 0.05, ***p < 0.001).

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