Figure 7
Isorhamnetin 3-O-neohesperidoside upregulated RANKL expression in bone crown-covered bone in the lower first molars of mice in vivo. The mouse mandible was separated (A1) and fresh crown coverage of dental follicle of the lower first molar was collected for western blotting (red arrow) (A2). (B) Positive RANKL expression was observed in the crown coverage of dental follicle by immunofluorescence. (C1) Many TRAP-positive osteoclasts were observed around the crown-covered bone in the control groups. (C2) Higher magnification of black-boxed regions in (C1). (D3) More TRAP-positive osteoclasts were detected in the isorhamnetin 3-O-neohesperidoside(I3ON)-treated groups (D1) than in the control groups at postnatal day 11. (D2) Higher magnification of black-boxed regions in D1. (E) Western blotting showed that isorhamnetin 3-O-neohesperidoside upregulated RANKL expression in the crown coverage of dental follicle. (F1) Unabsorbed crown-covered bone and several TRAP-positive osteoclasts were still observed in the control group at postnatal day 13. (F2) Higher magnification of black-boxed regions in F1. (G1) Crown-covered bone was completely resorbed and mucosal penetration (black arrow) was initiated in the isorhamnetin 3-O-neohesperidoside-treated groups at postnatal day 13. (G2) Higher magnification of black-boxed regions in G1. (*p < 0.05).
