Figure 2 | Scientific Reports

Figure 2

From: Enhanced purification coupled with biophysical analyses shows cross-β structure as a core building block for Streptococcus mutans functional amyloids

Figure 2

Amyloidophilic dye uptake assays. Ten micrograms of purified recombinant C123 (red bars), AgA (blue bars), or Smu_63c (green bars) were reacted with ThT, CDy11, BD-oligo, and MK-H4. Empty bars correspond to the purified monomeric proteins, solid bars to the samples after amyloid induction, and diagonal patterned bars to the purified amyloid fibers after treatment of the amyloid material to remove residual monomers. C123 and AgA polypeptides correspond to naturally occurring amyloidogenic derivatives of P1 and WapA, respectively. Arbitrary units (a.u.) ThT (Excitation = 440, Emission = 485), CDy11 and BD-Oligo (Ex = 530, Em = 580), and MK-H4 (Ex = 475, Em = 567). Protein concentration 0.1 mg/mL. Error bars represent standard deviations.

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