Figure 4 | Scientific Reports

Figure 4

From: In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells

Figure 4

Comparison of hiPSC-derived and isolated AEC2 cells. (A) Change of TEER over 2 weeks of culture at the air-liquid interface (n = 3 independent experiments from 2 cell lines). (B) Normalized expression of markers associated with AEC2 and AEC1 phenotype following 2 week culture at the air-liquid interface (n = 7). (C) Normalized expression alveolar associated markers following 2 weeks of culture at the air-liquid interface and following stimulation with CHIR99021 (n = 3). SFTPB/C/D = Surfactant protein B/C/D, AQP5 = Aquaporin 5, PDPN = Podoplanin. Data shown as mean; error bars represent SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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