Figure 1 | Scientific Reports

Figure 1

From: Assessment of unconventional antimicrobial compounds for the control of ‘Candidatus Liberibacter asiaticus’, the causative agent of citrus greening disease

Figure 1

Analysis of ‘Candidatus Liberibacter asiaticus’ gene expression following trunk injections with benzbromarone and tolfenamic acid. (a) A pneumatic trunk injection system was specifically designed to deliver trunk injections with accurate pressure control to minimize tissue damage. (b) Injectors connected to small citrus trees. (c) The expression of CLas 16S rRNA in leaf tissue, was measured by qRT-PCR in trees injected with buffer only (Control), benzbromarone (Benz), tolfenamic acid (Tolf), or benzbromarone and tolfenamic acid (Benz + Tolf). Leaf tissue samples were collected for analysis immediately before injections (▲), three months post-injection (■), and six months post-injection (●). ANOVA and Tukey’s post-hoc test was performed for each time point; only relevant statistical differences are shown. All expression data was normalized to the citrus COX gene.

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