Figure 4
Immunofluorescence staining and quantification of β1 integrin. (a) Rat livers were perfused with either norUDCA or TUDC (20 µmol/l each) for up to 15 min and immunostained for the active β1 integrin conformation and actin as given under “Experimental Procedures”. The scale bar corresponds to 20 µm. Representative pictures of three independent experiments are depicted. (b) β1 integrin fluorescence was quantified by using ImageJ analysis software. Whereas TUDC induced β1 integrin activation within 5 min, norUDCA activated β1 integrins with lower effect. *p < 0.05 denotes statistical significance compared with the unstimulated control; #p < 0.05 statistical significance between norUDCA and TUDC perfusion.
