Figure 1

Eml1 mRNA expression. (a) Expression of the long and short forms of Eml1 transcriptional variants, as detected by RT-PCR, indicates differential expression across body tissues and during embryonic development. RT-PCR of 18 S RNA served as a loading control. (b) Quantitative RT-PCR of all three Eml1 variants in Eml1^tvrm360 (tvrm360) mutants (n = 5) and C57BL/6J control whole eyes (n = 5) at postnatal day 14. Student’s t-test; *P < 0.0001. (c) Quantitative PCR of retina at postnatal day 14 was performed using primers specific to the long and short forms of Eml1 (n = 4 each group). Student’s t-test; *P < 0.05. Relative Eml1 mRNA levels ± standard error are shown in b,c. B6J values are shown in blue and Eml1^tvrm360 values are shown in red.