Figure 7
From: Disruption in murine Eml1 perturbs retinal lamination during early development
Displaced mitotic cells in developing retinas of homozygous Eml1tvrm360 mice. (a,b) Immunolocalization of mitotic cells in ocular sections with anti-PH3 antibody staining (red) in the NBL of Eml1tvrm360 mice (b) and control littermates (a) at birth (P0). Nuclei were counterstained with DAPI (blue). Boxed regions measure 300 µm per side. (c,d) The PH3 channel of the boxed regions in a and b, respectively, is presented in grayscale to visualize weakly stained cells. The selected boundaries of the apical NBL (red) and total NBL (yellow) are shown. (e) Quantitation of displaced mitotic cells from the apical boundary of the NBL in Eml1tvrm360 (n = 7) and control littermates (n = 6), including one heterozygous and five wild-type mice. Total PH3 + fluorescence at the apical NBL was divided by that of the entire NBL.
