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Figure 1

From: SPANX-A/D protein subfamily plays a key role in nuclear organisation, metabolism and flagellar motility of human spermatozoa

Figure 1

Characterisation of SPANX-A/D in human spermatozoa (A) Immunoblotting of SPANX-A/D protein in soluble and insoluble protein fractions (N = 5) (B) Immunofluorescence of SPANX-A/D protein in human spermatozoa. Negative controls were performed by omitting the primary antibody before secondary antiserum addition. Nuclei were stained with Hoechst and are represented in blue. Scale bar 5 μm (N = 4). (C) Immunoblotting of SPANX-A/D protein family in fertile (normozoospermic samples; F1: 750.000 cells; F2: 500.000 cells) and infertile (oligoteratoastenozoospermic sample; I:500.000 cells) patients. Tubulin was used as loading control. Representative image form 3 independent oligoteratoasthenozoospermic samples. (D) Table containing the unique peptides corresponding to each SPANX-A/D isoform found by LC-MS/MS in human spermatozoa. Comparison of each SPANX isoform sequence; each colour corresponds to the different peptides found by mass spectrometry. (E) Phosphorylation sites of each SPANX-A/D isoform found by LC-MS/MS are represented in yellow. The tree overlapping consensus NLSs are indicated in boxes.

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