Figure 1

Analysis of ACP electrophysiological parameters. (A) A total of 104 ACPs were recorded in the PmCo. Recorded cells were analyzed using three grouping variables: putative cell subtype, sex and age. (B) Two putative ACP subtypes were identified based on the relative complexity of apical and basal dendritic arbors and on their location relative to the PmCo layer II-III boundary. (C) Traces showing hyperpolarization (bottom traces), threshold (gray intermediate traces) and depolarization (top traces) responses of ACP pairs representative of the three categories: cell subtype, sex and age (current clamp protocol: injected current range, from −200 to +190 pA, step 10 pA, duration 600 ms). No differences in firing patterns or other electrophysiological parameters were observed among cells, except for those of different age. (D) Categorical principal component analysis (CATPCA) was used to identify factors underlying the variability of recording parameters based on cell type (E), sex (F) and age (G). The plot in D shows the variable loadings into the reduced dimensions individuated by CATPCA, in the rotated variable space (see Table 1 for the complete list of variables used). In the histogram the contribution of each grouping variable to the total variance of the sample is reported. (E–G) Recordings labeled by subtype (E), sex (F) and age (G). Gray data points in (E,F) were not part of the categorization.