Figure 5

AOB lesion decreases EPSCs and mEPSCs frequency in ACPs. Lesioning the AOB did not affect the amplitude of excitatory postsynaptic currents (EPSCs) recorded from ACPs (A,B) but produced a sensible decrease in their frequency (C). The amplitude/frequency distribution of EPSCs in 4 weeks old lesioned animals looks similar to 2 weeks old subjects (C). (D) 3D reconstruction (frontal view) of the main olfactory bulbs (MOB) of a lesioned (AOBX) and a sham operated subject (normal), showing the extent of the lesion in the former and an intact AOB in the latter, for comparison. AOB lesions have similar effects on miniature excitatory post-synaptic currents (mEPSCs, E): the average mEPSCs amplitude is unaffected (F) while the average frequency is differently distributed and reduced around the mean frequency range (G; Chi-square test, cross-tabulation 4 weeks vs 4 weeks-AOBX, df = 180 p < 0.001). (H) Scatter plots of the rise times and the amplitude of all mEPSCs recorded in 2-week (grey), 4-week (black) and 4-week-AOBX subjects showing no linear correlations (R-squared values are color coded on the right) indicate the absence of dendritic filtering (e.g. amplitude attenuation in slower events could indicate their distal origin). Scale bar (D), 500 μm. Sample sizes indicated in the first plot are the same for all quantifications. See Table 2 for the results of the statistical tests.