Figure 3 | Scientific Reports

Figure 3

From: Paraventricular, subparaventricular and periventricular hypothalamic IRS4-expressing neurons are required for normal energy balance

Figure 3The alternative text for this image may have been generated using AI.

IRS4PVH neurons project to hindbrain and spinal cord regions. (A) A Cre-dependent synaptophysin-mCherry adenovirus (syn-mCherry) was unilaterally injected in the PVH area of IRS4-iCre mice to trace synaptic terminals throughout the brain. (B,C) Syn-mCherry positive terminals are observed in hindbrain regions including the medial and lateral parabrachial nucleus (mPBN, lPBN, respectively, (B), as well as the dorsal motor nucleus of the vagus (DMV) and nucleus of the solitary tract (NTS, C). (D) IRS4PVH neurons also project to the median eminence (ME). (E) Syn-mCherry (red) identifies synaptic terminals in the intermediolateral column of the spinal cord (IML) in close proximity to neurons expressing choline acetyltransferase (ChAT, green). scp = superior cerebellar peduncle, 4 V = fourth ventricle, 3 V = third ventricle, AP = area postrema.

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