Figure 2
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
SMC marker expression after expansion in different media. (a) Histograms of flow cytometric surface marker expression (grey) of CD13, CD14, CD31, CD44, CD45, CD73, CD90, and CD140b on single live cells at passage three or four identified according to the gating strategy shown in Supplementary Fig. S2. Marker expression is given in comparison to the unstained control (white). (b) Gene expression level of SMC marker in isolated cells at passage one or two, normalized to the expression of the housekeeping gene RPL13A. Values of the individual donors are shown as points superimposed onto the boxplots. Expansion medium of each donor is given in brackets; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife.
