Figure 3
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
Contractility of SMC varies between donors. SMC expanded in different media were functionally assessed by stimulation with 10 µM carbachol for 15 min in (a) their respective expansion medium (given in brackets) or (b) after subcultivation in VascuLife medium for one passage. For visualization of stimulation-induced cell area change the membrane of single cells was marked; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife.
