Figure 4
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification

Proliferation of SMC varies between expansion media. SMC of passage three were seeded at 2 × 103 cells/well and expanded in different media. Cell proliferation was determined at the indicated time points using the CyQuant Proliferation assay and is shown as the fold change to day zero; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife.