Figure 5 | Scientific Reports

Figure 5

From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification

Figure 5The alternative text for this image may have been generated using AI.

Osteogenic stimulation elevates alkaline phosphatase activity and phosphate level indicating an osteogenic differentiation of SMC. (a) Alkaline phosphatase (ALP) activity after osteogenic stimulation of SMC in VascuLife, DMEM, or M199. ALP activity was normalized to the metabolic activity of the cells determined with PrestoBlue Cell Viability Reagent. (b) Phosphate (PO4) level determined in the supernatant of SMC osteogenically stimulated in VascuLife, DMEM, or M199. ALP and PO4 values are given as mean of five replicate wells per donor and medium ± standard deviation; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife.

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