Figure 6
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
Increased gene expression of osteogenic markers after stimulation in VascuLife and DMEM substantiates an osteogenic differentiation of SMC while SMC characteristics are partially maintained. Gene expression analysis of SMC osteogenically stimulated in VascuLife or DMEM for ten days; (a) osteogenic marker, (b) negative regulators of calcification, (c) SMC marker and transcriptional regulators. Expression was normalized to the expression of the housekeeping gene RPL13A and the fold change between day zero (baseline) and day ten was calculated. Expansion medium controls for DMEM and DMEM plus supplements were pooled for quantitative analysis. Gene expression analysis of ID2-derived SMC was not possible due to low cell numbers and resulting insufficient amounts of isolated mRNA. For statistical analysis a Kruskal-Wallis-test with an uncorrected Dunn’s post test was used. Only significant differences (p < 0.05) are given; D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife, EM - expansion medium, OM - osteogenic medium.
