Figure 7
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
SMC deposit calcified matrix only after osteogenic stimulation in DMEM. Alizarin red staining of calcified matrix deposited by SMC osteogenically stimulated in different culture media for 14 and 21 days. (a) Macroscopic examination of calcified matrix deposition by SMC after 21 days of osteogenic stimulation in VascuLife or DMEM. (b) Microscopic examination of the wells and their respective expansion media controls after 21 days of osteogenic stimulation in VascuLife or DMEM. (c) Quantification of the deposited calcified matrix normalized to the cell number on day 14 and day 21. Expansion medium controls for DMEM and DMEM plus supplements were pooled for quantitative analysis. Values of the individual donors are shown as points superimposed onto the boxplots and are given as mean of five replicate wells per donor and medium. For statistical analysis data of each point in time were compared amongst each other using a Kruskal-Wallis-test with a Dunn’s post test corrected for multiple comparisons. Only significant differences (p < 0.05) are given; D - DMEM, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife, EM - expansion medium, OM - osteogenic medium.
