Table 2 Osteogenic media and expansion medium controls used for comparison regarding the differentiation potential of SMC.

From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification

donor

expansion medium

control (EM)

osteogenic medium (OM)

VL

D

D+S

M

M+S

VL

D

M

ID1

x

x

   

x

x

 

ID2

x

x

   

x

x

 

ID3

x

x

   

x

x

 

ID4

x

 

x

  

x

x

 

ID5

x

x

 

x

 

x

x

x

ID6

x

x

  

x

x

x

x

  1. EM - expansion medium; OM - osteogenic medium (EM with 50 µM ascorbic acid, 10 mM β-Glycerophosphate and 0.1 µM dexamethasone); D - DMEM, M - M199, S - supplements (FGF, EGF, insulin, ascorbic acid), VL - VascuLife.
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