Table 3 List of antibodies used for SMC immunofluorescence and flow cytometry.
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
Antibody target | Clone | Conjugate | Dilution | Application | Company | Catalogue number (Lot) |
|---|---|---|---|---|---|---|
Anti-human SMA | 1A4 | PE | 1/50 | IF | R&D Systems | IC1420P (B174246) |
Anti-human MYH4 | MF20 | — | 1/250 | IF | eBioscience | 14-6503-82 (CAEI0818101) |
Anti-human MYH11 | 1G12 | — | 1/100 | IF | Abcam | ab683 (GR273859-7) |
Goat anti-mouse IgG (H + L) | Polyclonal | Alexa Fluor 488 | 1/400 | IF | Thermo Fisher Scientific | A-11001 (1572559) |
Anti-human CD13 | WM15 | APC-R700 | 1/400 | FC | BD Horizon | 565124 (7353767) |
Anti-human CD14 | MfP9 | FITC | 1/200 | FC | BD | 345784 (13599) |
Anti-human CD31 | WM59 | PE | 1/100 | FC | BD Pharmingen | 555446 (16944) |
Anti-human CD44 | IM7 | BV785 | 1/100 | FC | BioLegend | 103041 (B206615) |
Anti-human CD45 | HI30 | BV510 | 1/100 | FC | BioLegend | 368525 (B222302) |
Anti-human CD73 | AD2 | BV605 | 1/50 | FC | BD Horizon | 563199 (7026510) |
Anti-human CD90 | 5E10 | APC | 1/800 | FC | BioLegend | 328114 (B174246) |
Anti-human CD140b | 28D4 | PerCP/Cy5.5 | 1/50 | FC | BD Pharmingen | 562714 (7083965 & 722771) |
