Table 4 List of primers used for qRT-PCR.
From: Induced osteogenic differentiation of human smooth muscle cells as a model of vascular calcification
Gene | Abbre-viation | Direction | Sequences 5′ → 3′ | Reference ID |
|---|---|---|---|---|
Alkaline phosphatase, tissue-nonspecific | ALPL | Forward | ATGTTCCTGGGAGATGGGATG | — |
Reverse | ACCTGGGCATTGGTGTTGTA | |||
Aortic smooth muscle actin, alpha2 | ACTA2 | Forward | GCCAAGCACTGTCAGGAATC | — |
Reverse | GGGTACTTCAGGGTCAGGAT | |||
calcium-activated potassium channel subunit beta-1 | KCNMB1 | Forward | GAGAAGGTCAGAGCCAAATTCC | 25777641c2 |
Reverse | AATAGGACGCTGGTTTCGTTC | |||
Calponin 1 | CNN1 | Forward | GAACGTGGGAGTGAAGTACGC | 56676373c3 |
Reverse | CAGCCCAATGATGTTCCGC | |||
Collagen type I alpha 2 chain | COL1A2 | Forward | AGCCGGAGATAGAGGACCAC | — |
Reverse | GGCCAAGTCCAACTCCTTTT | |||
Krüppel-like factor 4 | KLF4 | Forward | CGGACATCAACGACGTGAG | 194248076c3 |
Reverse | GACGCCTTCAGCACGAACT | |||
Matrix gla protein | MGP | Forward | TCCGAGAACGCTCTAAGCCT | 299890878c1 |
Reverse | GCAAAGTCTGTAGTCATCACAGG | |||
Myocardin | MYOCD | Forward | ACGGATGCTTTTGCCTTTGAA | 226423888c1 |
Reverse | AACCTGTCGAAGGGGTATCTG | |||
Myosin heavy chain 11 | MYH11 | Forward | GGTCACGGTTGGGAAAGATGA | 92091584c2 |
Reverse | GGGCAGGTGTTTATAGGGGTT | |||
Osteopontin | SPP1 | Forward | CACTACCATGAGAATTGCAGTGA | — |
Reverse | CTGCTTTTCCTCAGAACTTCCA | |||
Platelet-derived growth factor receptor β | PDGFRB | Forward | TCTTTGTGCCAGATCCCACC | — |
Reverse | AGTGCAACGTCCCCTTTCTT | |||
Ribosomal protein L13a | RPL13A | Forward | CCTGGAGGAGAAGAGGAAAGAGA | — |
Reverse | TTGAGGACCTCTGTGTATTTGTCAA | |||
Runt related transcription factor 2 | RUNX2 | Forward | CTCCTACCTGAGCCAGATGA | — |
Reverse | CGGGGTGTAAGTAAAGGTGG | |||
Transgelin | TAGLN | Forward | GAAACCCACCCTCTCAGTCA | — |
Reverse | ATGTCTGGGGAAAGCTCCT |
