Figure 4
From: Cardiotrophin-1 Deficiency Abrogates Atherosclerosis Progression
Lack of CT-1 in Accelerated Atherosclerosis Improves Parameters Associated with Plaque Stability. Bar graphs represent the mean ± SEM of (a) Picrosirius Red quantification of total collagen, expressed as % of aortic root area; and (b) representative pictures of Picrosirius Red staining of total collagen in atherosclerotic roots in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and *p < 0.05 and **p < 0.01, expressed as % of aortic root area. The scale bar is 200 μm. (c) Bar graphs represent the mean ± SEM of necrotic core expressed as % of total aortic root area in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, and (d) representative pictures of necrotic core quantification in atherosclerotic roots in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and *p < 0.05, and ***p < 0.001 expressed as % of aortic root area. The scale bar is 200 μm. (e) Bar graphs represent the mean ± SEM of fibrous cap thickness in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, and (f) representative pictures of necrotic core thickness quantification in atherosclerotic roots in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and *p < 0.05, and ***p < 0.001 expressed as % of aortic root area. The scale bar is 200 μm. (g) Bar graphs represent the mean ± SEM of α-SMA expressed as % of total aortic root area in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, and (h) representative pictures of α-SMA staining in atherosclerotic roots in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and **p < 0.01 expressed as % of aortic root area. (i) Bar graphs represent the mean ± SEM of MMP9 expressed as % of aortic root area in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, and (j) representative pictures of MMP9 in atherosclerotic roots in Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and *p < 0.05, and ***p < 0.001 expressed as % of aortic root area. (k) Bar graphs represent the mean ± SEM of pro-MMP9 quantification in the serum of Apoe−/− or Apoe−/−ct-1−/− mice on NCD or HCD, as indicated, with n = 6–8/group and *p < 0.05. The scale bar is 200 μm.
