Table 2 Primers used in this work.
Primer name | Sequence 5′-3′ | Product |
|---|---|---|
∆gE-F | ATACTGCCGGCCAGACTACGGAACCTCAACAATTGGTACGTAGGGATAACAGGGTAATCGATTT | Kana gene flanked by homology arms of gE |
∆gE-R | TAACTATTTCACTAGTGAGTCATTAGTTCAACATCCATGACGTACCAATTGTTGAGGTTCCGTAGTCTGGCCGGCAGTATGCCAGTGTTACAACCAAT | |
RgE-F | TTGGAGTACTAAACACCAACATACTGCCGGCCAGACTACGGAACCTCAACAATTGGTACGATGATGGTTACTTTTATATC | gE gene flanked by homology arms of gE |
RgE-R | TGAGTCATTAGTTCAACATCCATGATCAGATGCGGAAACTAGATT | |
RgE-Kana-F | TCATGGATGTTGAACTAATGACTCACTAGTGAAATAGTTACCTGTATTACTAGGGATAACAGGGTAATCGAT | Kana gene flanked by homology arms of gE |
RgE-Kana-R | CAGGTGTCGGCCTAATATACCTGTGCATTAGTAATACAGGTAACTATTTCACTAGTGAGTCATTAGTTCAACATCCATGATGTTACAACCAATTAACCA | |
gE-F | TCTCAAGACGCTCTGGAATC | gE gene identification primers |
gE-R | AGCGAGTACTTCTCTGCGTC |
