Figure 3 | Scientific Reports

Figure 3

From: Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

Figure 3

Design of fusion nanobodies. (A) Overall modelled structure of the GFP-enhancer-GFPuv-LaG16 complex based on crystal structures of GFP-enhancer-GFP (PDB ID: 3K1K) and GFPuv-LaG16 (PDB ID: 6LR7). GFP-enhancer, GFPuv and LaG16 are coloured pink, green and blue, respectively. The distance from the C terminus of the GFP-enhancer to the N terminus of LaG16 and the distance from the N terminus of the GFP-enhancer to the C terminus of LaG16 were measured by PYMOL software. (B) Design of the vector carrying the two nanobodies and a linker. Different numbers of (GGGGS) repeats were inserted to connect the C terminus of the GFP-enhancer and the N terminus of LaG16, forming a fusion nanobody.

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