Figure 4
Analysis of the affinity of different nanobodies to GFPuv through ITC. The raw ITC data and fitted curves are shown. The data were obtained by injecting (A) LaG16, (B) GFP-enhancer, (C) GGGGS4 (GFP-enhancer-(GGGGS)4-LaG16), (D) GGGGS5 (GFP-enhancer-(GGGGS)5-LaG16), or (E) GGGGS6 (GFP-enhancer-(GGGGS)6-LaG16) into GFPuv in the cell.
