Figure 5 | Scientific Reports

Figure 5

From: Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

Figure 5

Analysis of zfP2X4 purification using nanobody-coupled resin or TALON resin through SDS-PAGE gel. The first sample line shows the solubilization solution of the cell membrane, containing all kinds of membrane proteins. The numbers on the left indicate the molecular weight of the marker. The 3rd, 4th and 5th lines are the protein purified using TALON, GGGGS4 (GFP-enhancer-(GGGGS)4-LaG16)-coupled and GFP-enhancer-coupled resins, respectively. The bands for GFP-zfP2X4 are indicated on the right.

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