Table 1 The binding specificity of different nanobodies to GFPuv.

From: Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

 

n

ΔΗ° (kcal/mol)

−TΔS° (kcal/mol)

ΔG° (kcal/mol)

KD (nM)

GFP-enhancer

0.99 ± 0.02

−16.0 ± 0.7

5.5

−10.5 ± 0.7

24.3 ± 17.0

LaG16

0.99 ± 0.03

−9.1 ± 0.3

2.0

−7.1 ± 0.3

6.7 ± 14.5

GGGGS4

0.99 ± 0.02

−23.7 ± 0.8

10.9

−12.8 ± 0.8

0.5 ± 2.3

GGGGS5

1.01 ± 0.01

−23.3 ± 0.4

10.6

−12.7 ± 0.4

0.6 ± 0.6

GGGGS6

1.1 ± 0.01

−22.1 ± 0.4

9.8

−12.3 ± 0.4

1.2 ± 0.7

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