Table 2 Purification of GFP-tagged zfP2X4 from 7.67 ml of cultured cells.

From: Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

 

Total Protein (μg)

Purity (%)

Concentration (mg/ml)

Volume (μl)

Yield (μg)

TALON

32.5

36.3

0.650

50

11.8

GGGGS4

10.7

69.3

0.214

50

7.42

GFP-enhancer

6.75

75.2

0.135

50

5.08

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