Table 4 Data collection, phasing and refinement statistics.

From: Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

Data collection

Wavelength (Å)

1.000

Space group

P21

Cell dimensions

a, b, c (Å)

48.4, 41.8, 81.7

α, β, γ (°)

90, 91.9, 90

Resolution (Å)*

42.22–1.67 (1.73–1.67)

Rsym*

0.127 (0.609)

I/σI*

11.6 (2.04)

Completeness (%)*

99.34 (94.21)

Redundancy*

7.0 (3.9)

CC1/2 (%)*

99.4 (70.8)

Refinement

Resolution (Å)

1.67

No. reflections

37934

Rwork/Rfree

20.5/23.8

No. atoms

Protein

2883

Ligands

22

Solvent

170

B-factors

Protein

13.2

Ligands

12.6

Solvent

27.0

R.m.s. deviations

Bond lengths (Å)

0.024

Bond angles (°)

1.8

Ramachandran plot

Favoured (%)

99.15

Allowed (%)

0.85

Outliers (%)

0

  1. *The highest resolution shell is shown in parentheses.
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