Figure 5

OsPLDα1 transcript levels in immature seeds from wild Oryza species accessions. Mean values for OsPLDα1 transcripts and standard deviation (S.D.) measured relative to Actin expression. Relative transcript levels of OsPLDα1, in accessions of wild Oryza species, for four qRT-PCR primers namely PLDE1 (designed from first exon of the gene), PLDE2.1 (designed from 5′end of second exon), PLDE2.2 (designed from 3′end of second exon), and PLDE3 (designed from third exon of the gene) are shown. Among all the wild species accessions, IR101152 accession of O. officinalis was found to have lowest transcript levels using all the four qRT-PCR primers.