Table 3 Amino acid variations among 20 OsPLDα1 variants.

Protein^a	Ref.^c	I	II	III	IV	V	VI	VII	VIII	IX	X	XI	XII
Variant^b	1, 4, 8	2	3	5, 6, 7	9	10	11	12	13	14	15, 16, 17	18	19, 20
76	I	I	I	I	I	I	I	I	I	I	V	V	V
77	N	N	N	N	N	N	N	N	N	N	D	N	N
128	R	R	R	R	R	R	R	R	T	T	T	T	T
139	P	P	P	P	P	P	P	P	P	P	P	L	P
159	N	N	N	N	N	N	N	N	K	N	N	N	N
165	R	R	H	R	R	R	R	R	R	R	R	R	R
239	E	E	E	K	E	E	E	E	E	E	E	E	E
243	V	V	V	V	V	V	V	V	I	V	V	V	V
299	E	E	E	E	E	E	E	E	A	A	A	A	A
306	D	D	D	D	N	D	D	D	D	D	D	D	D
320	G	G	G	G	G	G	G	G	S	G	G	G	G
345	L	L	L	L	L	L	L	L	L	L	F	L	L
346	P	P	P	P	P	P	P	P	P	P	A	P	P
347	N	N	N	N	N	N	N	N	N	N	K	N	N
348	Q	Q	Q	Q	Q	Q	Q	Q	Q	Q	P	Q	Q
350	S	S	S	S	S	S	S	S	S	S	L	S	S
351	Q	Q	Q	Q	Q	Q	Q	Q	Q	Q	P	Q	Q
352	Q	Q	Q	Q	Q	Q	Q	Q	Q	Q	T	Q	Q
353	R	R	R	R	R	R	R	R	R	R	K	R	R
372	Q	Q	Q	Q	Q	Q	Q	Q	Q	Q	E	Q	Q
373	Y	Y	Y	Y	Y	Y	Y	Y	Y	Y	D	Y	Y
374	H	H	H	H	H	H	H	H	H	H	S	H	H
375	S	S	S	S	S	S	S	S	S	S	Q	S	S
377	F	F	F	F	F	F	F	F	F	F	R	F	F
378	R	R	R	R	R	R	R	R	R	R	W	R	R
395	T	T	T	T	T	N	N	N	N	N	N	N	N
399	K	N	K	K	K	K	K	K	T	K	T	T	T
481	P	P	P	P	P	P	P	P	L	P	P	P	P
634	A	A	A	A	A	A	G	A	G	G	G	A	A
728	M	M	M	M	M	M	M	T	M	T	T	T	T

Amino acids (indicated with the letter code) and their positions (indicated in numbers) in the OsPLDα1 variants. Amino acids with highlighted background show substitutions in OsPLDα1 protein variants in comparison to the OsPLDα1 protein (AB571657.1).
^aGroups of OsPLDα1 variants based on amino acid sequences.
^bGroups of OsPLDα1 variants based on nucleotide sequences.
^cRef indicate the nucleotide sequences variants which translates into the amino acid sequence same as that of the reference OsPLDα1 protein sequence of Nipponbare.

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