Table 1 Box-Behnken factorial experimental design for pigment formation by Natrialba sp. M6.

From: In vitro dual (anticancer and antiviral) activity of the carotenoids produced by haloalkaliphilic archaeon Natrialba sp. M6

Trial

NaCl

pH

Inoculum age

Response*

Pigment conc. g/L

1

0 (20)

0 (10)

0 (9)

1.176

0.7867

2

0 (20)

0 (10)

0 (9)

1.183

0.7915

3

0 (20)

−1 (9)

1 (11)

0.59

0.3885

4

0 (20)

1 (11)

−1 (7)

0.83

0.5516

5

−1 (15)

−1 (9)

0 (9)

0.98

0.6535

6

0 (20)

0 (10)

0 (9)

1.189

0.7955

7

1 (25)

0 (10)

−1 (7)

1.032

0.6889

8

0 (20)

1 (11)

1 (11)

1.152

0.7704

9

0 (20)

−1 (9)

−1 (7)

1.011

0.6746

10

−1 (15)

0 (10)

1 (11)

0.39

0.2527

11

−1 (15)

0 (10)

−1 (7)

0.79

0.5244

12

1 (25)

0 (10)

1 (11)

1.51

1.0136

13

1 (25)

1 (11)

0 (9)

0.83

0.5516

14

1 (25)

−1 (9)

0 (9)

1.009

0.6732

15

−1 (15)

1 (11)

0 (9)

0.34

0.2187

  1. *Response is the absorbance of the pigments after extraction measured at a wavelength of 350 nm.
  2. Coded values: (−1) low level; (+1) high level and (0) middle level.
  3. Levels of studied variables presented between brackets are expressed in terms of (%wt/v), value and days for X1, X2 and X3, respectively.
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