Figure 1
From: RNA-Seq of human whole blood: Evaluation of globin RNA depletion on Ribo-Zero library method
Experimental Design and Analysis. (a) Blood RNA preparation steps from 6 human subjects: 3 controls (c) and 3 patients (P). RNA was used for preparing libraries with Ribo-Zero Gold (RZG) or Globin-Zero (GZ) kits as shown. In Experiment 1, a total of 33 libraries were prepared with either 250 ng total RNA for low (L) input or 900 ng for high (H) input. In Experiment 2 technical replicate libraries were prepared using 250 ng RNA from C1 and C2 with either RZG or Globin-Zero for a total of 12 libraries. Individual RNA-seq data files generated from the libraries shown in B and C were named according to the following convention: sample ID_RNA input_library method_replicate number, e.g., C1_L_GZ_1. (b) Fastq files were assessed for quality metrics, aligned and analyzed as shown. Correspondence between data analysis steps and results shown in Figs. 2–4 is indicated.
