Figure 4
Effect of CaCl2 and SNP on the (a) chlorophyll a fluorescence OJIP transient curves and (b) spider plots for OJIP parameters inferred from chlorophyll a fluorescence OJIP transient in the leaves of As‒challenged Brassica seedlings subjected to different modulators. Data signifies the mean ± standard error of five replicates. Bars followed by different letters are significantly different at p < 0.05 level according to Tukey test. (c). The leaf model representing phenomenological energy fluxes per excited cross section (CS) in the leaves of As‒challenged Brassica seedlings subjected to different modulators. [(I) Control, (II) As, (III) As + Ca, (IV) As + Ca + EGTA, (V) As + Ca + EGTA + LaCl3, (VI) As + Ca + c‒PTIO, (VII) As + Ca + L‒NAME, (VIII) As + Ca + c‒PTIO + L‒NAME, (IX) As + SNP, (X) As + SNP + c‒PTIO and (XI) As + SNP + L‒NAME]. The relative value for the measured parameters is the mean of quintuplicates (n = 5). The width of arrow corresponds to the intensity of flux parameters; ABS/CS: absorption flux per CS, TR0/CS: trapped energy flux per CS, ET0/CS, electron transport flux per CS and DI0/CS: dissipated energy flux per CS. Circles embedded in circle (RC/CS) are percentage of active/inactive RCs, where white circles are representing reduced QA RCs (active) and red circles non‒reducing QA RCs (inactive). RCs: reaction centres as described by Sitko et al.62.
