Figure 1
Design and production of the MyoTACTIC platform. (a) MyoTACTIC production started with creating a three-dimensional Computer Aided Design (3D CAD) using SolidWorks™ Software which was then printed using a ProJet MJP 3500 3D printer from 3D SYSTEMS. Next, a negative PDMS mold was created from the 3D printed part which was subsequently used to fabricate a soft replica of the design after silanizing. Finally, a rigid negative polyurethane mold was created from the PDMS replica which was used to fabricate multiple PDMS plates. (b,c) Computer generated 3D images of (b) MyoTACTIC 96-well plate design and (c) a cross-section of wells indicating the location of the micro-posts. (d) Schematic overview of human cell isolation and subsequent generation of hMMTs in MyoTACTIC. (e) Stitched phase-contrast image of 9 wells of MyoTACTIC containing remodeled hMMTs 10 days post seeding. Scale bar 5 mm. (f,g) Impact of micro-post design on formation and long-term maintenance of hMMTs in MyoTACTIC. Representative images of (f) collapsed and (g) successfully remodeled hMMTs seeded in wells with (f) hook-less and (g) hook featured posts. Micro-posts are outlined in yellow dashed lines. Red arrows indicate collapsed hMMTs on the top right panels and hMMTs are outlined in white dashed lines on the bottom right panels. Scale bars 500 µm.
